He was pretty shockingly an entrepreneur and inventor in all the best ways,’in a field dominated by very cautious scientists (who are great too, but who likely never would have gotten the genome sequenced within 10-20 years of when he did it). It was basically the Apollo Project in a field which was more like 1980s NASA in culture.
iiuc it was hamilton smith who insisted that shotgun sequencing would work. the nih side insisted on primer walking until celera started assembling the genome so rapidly that the nih had to get in on shotgun too
I belive you are mixing assembling the genome by combining sequences of individual, overlapping inserts of cosmids, fosmids, PACs and BACs (bacterial vectors with human DNA inserts of 40-150kbp) to whole genome shotgun. The inserts of the above bacterial vectors were sequenced using shotgun, but the gaps in the sequence were closed with custom primers.
No, at initial release, the human genome from the NIH side was done by bac-to-bac, not by shotgun.
> in a field dominated by very cautious scientists (who are great too, but who likely never would have gotten the genome sequenced within 10-20 years of when he did it).
I did a bio undergrad and one of my professors was involved. She was adamant that the Human Genome Project finished ahead of Celera and that the HGP published reference data that Venter and team fundamentally relied upon to even have their shotgun approach work.
i worked for ham smith and my understanding through him is that both sides relied on data that the other produced.
here are technical details, both were more or less independent, the celera sequence did include data from the other side as useful reference points but the assembly would have happened without it. https://pmc.ncbi.nlm.nih.gov/articles/PMC123615/