this is why you enforce a no brood condition by removing brood, and by rehiving your split into a new hive,with no brood.
the original hive can be kept as a queenless hive, and they will make replacement queens, if you keep a frame of brood.
if you get a gravid queen from the original hive, you can force broodless period when rehiving to a clean hive, and both sides of the split greatly reduce mite population.
if you have year round brood conditions, these maneuvers will be essential to mitigating varroa. you should also look at oxalic acid[careful!] as a mite deterrent.
this is why you enforce a no brood condition by removing brood, and by rehiving your split into a new hive,with no brood.
the original hive can be kept as a queenless hive, and they will make replacement queens, if you keep a frame of brood.
if you get a gravid queen from the original hive, you can force broodless period when rehiving to a clean hive, and both sides of the split greatly reduce mite population.
if you have year round brood conditions, these maneuvers will be essential to mitigating varroa. you should also look at oxalic acid[careful!] as a mite deterrent.