At very low doses, for example chewing the leaves of coke instead of using the high purified version, it's somewhat like drinking a coffee [1].
I expect the fish to be more active. A coffee patch would be a nice 4th group as another control.
[1] Chewing the leaves of coke is common in many countries of South America, https://en.wikipedia.org/wiki/Acullico
Coffee can do strange things to animals. There's a study where NASA gave various drugs to spiders to see how it affected their webs[0]. Coffee had a stranger effect on the web than marijuana.
[0] https://web.archive.org/web/20210327150247/https://arachnidl...
Salient point:
Caffeine is a chemical that plants evolved multiple times independently as an insecticide.
More than anything this seems like a good reminder that spiders aren't human.
And that can be easy to forget in this fast paced world.
Obligatory reference to this classic wildlife film from the Canadian Wildlife Service in Ottawa.
It's almost unbelievable.
https://www.youtube.com/watch?v=p2HipedgM3I
[flagged]
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Because even in low concentrations, I expect cocaine to have a different effect than Valium. (And in both case I expect a different effect at high concentrations.)
What the fuck is your problem?