I don't really understand how coffee, lacking heavy metals, can effectively give contrast in the electron microscope. I can't access the paper but the available parts didn't seem to explain how this works.
I don't really understand how coffee, lacking heavy metals, can effectively give contrast in the electron microscope. I can't access the paper but the available parts didn't seem to explain how this works.
Even without any heavy metal staining, you would end up seeing some structures. This approach allows looking at unstained / native tissue.
What is the coffee doing?