Depends on how clear it is.

I usually write code to help local debug-ability (which seems rare). For example, this allows one to trivially set a conditional breakpoint and look into the full response:

    response = get_response()
    response = response.json()

The fact that the first response is immediately overwritten proves to the reader it's not important/never used, so they can forget about it, where a temp variable would add cognitive load since it might be used later.

and I think is just as clear as this:

    response = get_response().json()

This motivated by years of watching people through code, and me working with deeply non-software engineers, and is always very much appreciated.

    get_response().json() 

    raw_response = get_response()
    response = raw_response.json()